<i>In-Vitro</i> Micropropagation and Acclimatization of an Endangered Native Orchid Using Organic Supplements

In-vitro propagation is a technique that provides vital solution for the conservation of endangered orchid species. The media used in tissue culture can be modified through addition inexpensive organic materials as an alternative to expensive synthetic additives. Some sources, such coconut water and fruit juice, contain significant amounts vitamins, amino acids, compounds which act growth regulators, making these sources excellent additives in-vitro cultivation. aim this study was develop protocol micropropagation acclimatization Epidendrum nocturnum using supplements various substrates at stage. Banana powder, water, potato dextrose were added basal seed sowing evaluated germination percentage plantlet growth. In addition, coir, horticultural charcoal, sphagnum moss, wood bark height, number leaves, shoots portion study. medium with showed greater (71.00% 76.75%) compared control (37.50% 45.50%) 60 90 days after sowing, respectively. Media values plant length roots control. combination coir charcoal shown more efficient than bark, results height leaves 30, 90, 120 transplantation E. nocturnum.